Pig Interleukin 1alpha, IL-1alpha ELISA Kit from MyBioSource.com

Supplier Page

Supplier Page from
MyBioSource.com for
Pig Interleukin 1alpha, IL-1alpha ELISA Kit

Get Pricing

Description

For SampleFor the quantitative determination of pig interleukin 1? (IL-1?) concentrations in serum, plasma, cell culture supernates, tissue homogenates.

PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL-1? has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-1? present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-1? is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-1? bound in the initial step. The color development is stopped and the intensity of the color is measured